|
課程名稱 |
次世代定序分析
Next Generation Sequencing Analysis |
|
開課學期 |
111-2 |
|
授課對象 |
生命科學院 基因體與系統生物學學位學程 |
|
授課教師 |
林友瑜 |
|
課號 |
GenSys5024 |
|
課程識別碼 |
B48EU0390 |
|
班次 |
|
|
學分 |
3.0 |
|
全/半年 |
半年 |
|
必/選修 |
選修 |
|
上課時間 |
星期二2,3,4(9:10~12:10) |
|
上課地點 |
|
|
備註 |
本課程以英語授課。地點:生科院3A教室
總人數上限：30人
外系人數限制：15人 |
|
|
|
|
課程簡介影片 |
|
|
核心能力關聯 |
核心能力與課程規劃關聯圖 |
|
課程大綱
|
|
為確保您我的權利,請尊重智慧財產權及不得非法影印
|
|
課程概述 |
During the past decade, large sequence data sets generated by next generation sequencing (NGS) has become the mainstream method to obtain and study genomic information. This course will cover the concept, methodology, algorithms, and current advances and tools for handling NGS generated data sets.
[Classroom changed to 4A] |
|
課程目標 |
Upon completion of the course, students should gain a more comprehensive understanding of the overall process of next generation sequencing starting from sample preparation to data analysis. |
|
課程要求 |
Homework assignments 30%
Midterm exam 35%
Final exam 35%
Discussions (in class or on COOL) Bonus |
|
預期每週課後學習時數 |
|
|
Office Hours |
|
|
指定閱讀 |
|
|
參考書目 |
|
|
評量方式
(僅供參考) |
|
|
週次 |
日期 |
單元主題 |
|
Week 1 |
2/21 |
Introduction |
|
Week 2 |
#2/28 |
###Peace Memorial Day### |
|
Week 3 |
3/07 |
Principles of next generation sequencing |
|
Week 4 |
3/14 |
NGS archives, quality control and normalization |
|
Week 5 |
3/21 |
Data assembly, alignment, and annotation |
|
Week 6 |
3/28 |
Detection and comparison of mutational profiles |
|
Week 7 |
#4/04 |
###Ching Ming Festival### |
|
Week 8 |
4/11 |
Comparison of expression profiles |
|
Week 9 |
4/18 |
Midterm Exam |
|
Week 10 |
4/25 |
Liquid biopsy |
|
Week 11 |
5/02 |
Single cell sequencing |
|
Week 12 |
5/09 |
Genome wide association studies |
|
Week 13 |
5/16 |
Epigenetic modification analyses |
|
Week 14 |
5/23 |
Splicing, viral integrations and genomic structural variations
Incorporation and development of analytic pipelines
Current research focus of iconic NGS studies
Bioinformatic tools for NGS analyses (online) |
|
Week 15 |
5/30 |
Visit to TCM |
|
Week 16 |
6/06 |
(No class) All assignments and final report due on 6/6. |
|